期刊论文

中文

农业环境科学学报

1672-2043

2014

33

5

1050-1056

10.11654/jaes.2014.05.031

863)(2012AA06A304:国家高技术研究发展计划（863计划） 2011467017:公益性行业科研专项重大项目

本校为第一机构

通过对样品预处理过程优化、提取剂与洗脱剂的筛选和检测色谱条件优化,建立了基于固相萃取-高效液相色谱荧光检测法分析鸡粪中6种氟喹诺酮类(FQs)的检测方法。该方法选用Mcllvaine-Na_2EDTA缓冲液∶乙腈(1∶1,V/V)(pH=2)作为提取剂,超声提取时间15 min,经HLB固相柱净化,选用甲醇为洗脱剂,浓缩后用HPLC/FLD检测,采用乙腈/0.7%磷酸作为流动相。FQs在0.01~1.0 mg·kg~(-1)浓度范围内呈现良好线性关系,R~2为0.996 9~0.999 9。加标(浓度水平0.01、0.5、1.0 mg·kg~(-1)干重)回收率达76.7%~106.7%,相对标准偏差0.7%~14.4%(n=4)。方法检出限为0.002~0.022 mg·kg~(-1),方法定量限为0.006 8~0.074 mg·k...

An analytical method for simultaneous determination of six fluoroquinolones（FQs ） in chicken manures based on solid-phase extraction and high performance liquid chromatography with a fluorescence detection （HPLC/FLD） was developed in the current study. Samples were ultrasonically extracted in Mcllvaine-Na2EDTA buffer/acetonitrile（ 1：1 ）（pH=2） for 15 min. The extract was cleaned by a HLB solid-phase extraction column, and then eluted with methanol solvent. The fluoroquinolones in the extract were determined by HPLC/FLD with acetonitrile...