The coding sequence of Mx1 gene of Carassius auratus was cloned under the control of carp β-actin gene promoter by molecular restructuring,then an all-fish Mx gene recombinant was constructed.The recombinant gene was transferred into fertilized zygotes of grass carps by micro-injection.A group of all-fish transgenic grass carps were obtained.Polymerase chain reaction(PCR) technology and Southern blot were carried out on treated grass carps fry(two months).It verified that all-fish Mx gene had integrated into the genome of grass carps....