期刊论文

中文

水生态学杂志

1674-3075

2013

34

3

94-100

10.3969/j.issn.1674-3075.2013.03.016

国家自然科学基金面上项目（31272652） 国家“863”计划（2011AA100404）

本校为第一机构

以赤眼鳟生理失活精子诱导的雌核发育草鱼F1群体(CC)和与之母本同源的普通草鱼群体(PC)为材料,采用RAPD和SCAR分子标记相结合的技术,在100条RAPD随机引物扩增的16条有效引物中扩增筛选出7条群体差异条带,其中1条为异精雌核发育草鱼群体中特有,其余均表现为异精雌核发育草鱼群体缺失;对7条RAPD特异条带的回收中仅得到S32和S336的特异条带.经回收、克隆、测序后根据序列信息设计了4对SCAR引物,其中S336的2对SCAR引物在两群体间扩增出的条带一致,特异性消失;S32的2对SCAR引物能扩增出两群体间的特异条带;对S32-Scar1 E/S32-Scar1R扩增出的SCAR标记S321643进行大样本检测结果表明:该标记在异精雌核发...

The genomic DNA from common grass carp （PC） and allogynogenesis grass carp F1 （CC） induced by inactivate sperms of squaliobarbus curriculus were isolated. Using RAPD and SCAR technology, 16 primers with high repeatability and stability were selected from the 100 RAPD random primers, and 7 specific DNA fragments had been discovered. One of the fragments was specific in allogynogenesis grass carp F1, while the others only appeared in common grass carp variety. After purification, cloning and sequencing, only two specific fragments amplified b...