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Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus.

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成果类型:
期刊论文
作者:
Hu, Kui;Qiu, Lin;Ding, Wenbing;He, Hualiang;Li, Youzhi*
通讯作者:
Li, Youzhi
作者机构:
[Hu, Kui; Qiu, Lin; He, Hualiang; Li, Youzhi] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China
[Ding, Wenbing; Li, Youzhi] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China
[Ding, Wenbing] Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China
通讯机构:
[Li, Youzhi] H
Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China. Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Southern rice black-streaked dwarf virus;Reference genes;qRT-PCR;Normalization;Isoprenoid metabolic pathway
期刊:
Molecular Biology Reports
ISSN:
0301-4851
年:
2019
卷:
46
期:
4
页码:
3945-3953
基金类别:
Funding This work was funded by the National Natural Science Foundation of China (31572005) and Research Foundation of Education Bureau of Hunan Province, China (15A090).
机构署名:
本校为第一且通讯机构
院系归属:
园艺园林学院
植物保护学院
摘要:
The Southern rice black-streaked dwarf virus (SRBSDV), a novel Fijivirus, poses a major threat to rice production in East Asia. Analysis of the gene expression of SRBSDV-infected rice plants may reveal the molecular basis of interactions between the virus, its vector and rice plants. Reliable reference genes are required for accurate qRT-PCR analysis. However, no reliable, valid reference genes for examining gene expression in SRBSDV-infected rice plants have so far been identified. We examined the expression of eight candidate reference genes in the leaves of SRBSDV-infected, and healthy, ric...

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