Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns of WRKY1 Transcription Factor and Rhynchophylline Biosynthesis-Related Genes in Uncaria rhynchophylla

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成果类型：

期刊论文

作者：

Mu, Detian;Shao, Yingying;He, Jialong;Zhu, Lina;Qiu, Deyou;...

通讯作者：

Lu, Ying;Tang, Q

作者机构：

[Zhou, Yu; Tang, Qi; Shao, Yingying; Lu, Ying; He, Jialong; Zhu, Lina; Mu, Detian; Lu, Y; Zhang, Yao; Tang, Q] Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.

[Qiu, Deyou] Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China.

[Wilson, Iain W.] Commonwealth Sci & Ind Res Org CSIRO, Agr & Food, Canberra, ACT 2601, Australia.

[Pan, Limei] Guangxi Bot Garden Med Plants, Key Lab Guangxi High Qual Format & Utilizat Dai di, Nanning 530023, Peoples R China.

[Tang, Qi; Tang, Q] Hunan Univ Med, Hunan Prov Key Lab Synthet Biol Tradit Chinese Med, Changsha 410208, Peoples R China.

通讯机构：

[Lu, Y; Tang, Q ] H

Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.

Hunan Univ Med, Hunan Prov Key Lab Synthet Biol Tradit Chinese Med, Changsha 410208, Peoples R China.

语种：

英文

关键词：

Uncaria rhynchophylla;RT-qPCR;reference gene;terpenoid indole alkaloids;expression pattern

期刊：

International Journal of Molecular Sciences

ISSN：

1661-6596

年：

2023

卷：

期：

页码：

16330-

DOI：

10.3390/ijms242216330

基金类别：

This work was supported by the Project of Hunan Natural Science Foundation (2022JJ30305), Guangxi Major Science and Technology Project of China (GuikeAA22096021), the Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine (No: HCSW2023-01), the Key Scientific Research Fund of Hunan Provincial Education Department (21A0138), the Horticulture’s Open Program of Hunan Agricultural University (2021YYXK002), the Postgraduate Scientific Research Innovation Project of Hunan Province (CX20220689), and the Postgraduate Scientific Research Innovation Project of Hunan Agriculture University (2022XC062).

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

Uncaria rhynchophylla (Miq.) Miq. ex Havil, a traditional medicinal herb, is enriched with several pharmacologically active terpenoid indole alkaloids (TIAs). At present, no method has been reported that can comprehensively select and evaluate the appropriate reference genes for gene expression analysis, especially the transcription factors and key enzyme genes involved in the biosynthesis pathway of TIAs in U. rhynchophylla. Reverse transcription quantitative PCR (RT-qPCR) is currently the most common method for detecting gene expression levels due to its high sensitivity, specificity, reprod...

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Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns of WRKY1 Transcription Factor and Rhynchophylline Biosynthesis-Related Genes in Uncaria rhynchophylla

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