期刊论文

Yao, Y.

中文

微生物学报

0001-6209

2007

47

5

805-809

10.3321/j.issn:0001-6209.2007.05.012

国家“973项目”（2005CB523002） 国家科技支撑计划资助项目（2006BAD06A04,2006BAD06A03）

本校为第一机构

从广东省疑似流感发病猪分离到1株H3N2亚型猪流感病毒(A/Swine/Guangdong/01/2005(H3N2)),对其各个基因进行克隆与测序,并与GenBank中收录的其它猪流感、禽流感和人流感的相关基因进行比较,结果表明,HA全基因与广东2003～2004 年分离的H3N2猪流感毒株的核苷酸序列同源性在99%以上,与纽约90年代末分离的H3N2人流感毒株同源性在98.5%以上;NA基因与纽约1998～2000 年分离的H3N2人流感毒株的核苷酸序列同源性在99%以上;NS基因、M基因的核苷酸序列与H1N1亚型猪流感毒株A/swine/HongKong/273/1994(H1N1)的核苷酸序列同源性较高,分别为97.9%、98.4%,与美洲A/swine/Iowa/17672/1988(H1N1)的核苷酸序列同...

An H3N2 subtype swine influenza virus, A/Swine/ Guangdong/01/2005 (H3N2), was isolated from pigs with influenza-like signs in Guangdong province in 2005. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the gene segments for sequencing analysis. Phylogenetic analysis showed that the hemagglutinin(HA) gene of A/Swine/ Guangdong/01/2005 shared high degree of sequence identity with those of H3N2 viruses isolated from swine in Guangdong province from 2003 to 2004 and H3N2 viruses isolated from human in New York in the end of 1990s. The neuraminidase (NA) gene of this H3...