In order to simplify the method for isolation and cultivation of anaerobic bacteria and culture them to form single colonies on solid culture medium under ordinary experimental laboratory,the volume of cooked meat broth was increased,and used as anaerobic multiple proportional dilute solution.The multiple proportional dilutions of anaerobic bacteria samples were performed under agar column.The anaerobic agar plate with a tiny hole at the cover sealed by a rubber plug was pre-reduced,injected and inoculated with multiple proportionally diluted bacterial solutions.The anaerobic effect of the who...
