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L-theanine protects H9C2 cells from hydrogen peroxide-induced apoptosis by enhancing antioxidant capability

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成果类型:
期刊论文
作者:
Li, Chengjian;Yan, Qiongxian*;Tang, Shaoxun;Xiao, Wenjun;Tan, Zhiliang
通讯作者:
Yan, Qiongxian
作者机构:
[Tang, Shaoxun; Yan, Qiongxian; Tan, Zhiliang; Li, Chengjian] Chinese Acad Sci, CAS Key Lab Agroecol Proc Subtrop Reg, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Stn Anim Nutr & Feed Sci,Minist Agr,Inst Subtrop, Changsha, Hunan, Peoples R China.
[Li, Chengjian] Xiangnan Univ, Coll Pharmaceut Sci, Chenzhou, Hunan, Peoples R China.
[Li, Chengjian] Yongzhou Vocat Tech Coll, Dept Pharm, Yongzhou, Hunan, Peoples R China.
[Yan, Qiongxian; Xiao, Wenjun] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha, Hunan, Peoples R China.
[Tang, Shaoxun; Tan, Zhiliang] CICAPS, Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.
通讯机构:
[Yan, Qiongxian] C
[Yan, Qiongxian] H
Chinese Acad Sci, CAS Key Lab Agroecol Proc Subtrop Reg, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Stn Anim Nutr & Feed Sci,Minist Agr,Inst Subtrop, Changsha, Hunan, Peoples R China.
Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
caspase 3;fluorescein isothiocyanate;glutathione;glutathione peroxidase;hydrogen peroxide;lipocortin 5;mitogen activated protein kinase p38;nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase;nitric oxide;penicillin derivative;reactive oxygen metabolite;streptomycin;stress activated protein kinase;superoxide dismutase;theanine;antioxidant;Casp3 protein, rat;glutamic acid derivative;glutathione;glutathione peroxidase;hydrogen peroxide;protective agent;superoxide dismutase;theanine;animal cell;antioxidant activity;apoptosis;Article;cell culture;cell damage;cell proliferation;cell structure;cell survival;cell viability;controlled study;flow cytometry;fluorescence microscopy;heart disease;nonhuman;oxidative stress;protein expression;protein phosphorylation;rat;reperfusion injury;signal transduction;upregulation;Western blotting;animal;apoptosis;cardiac muscle cell;cell line;drug effect;MAPK signaling;metabolism;phosphorylation;Animals;Antioxidants;Apoptosis;Caspase 3;Cell Line;Cell Survival;Glutamates;Glutathione;Glutathione Peroxidase;Hydrogen Peroxide;JNK Mitogen-Activated Protein Kinases;MAP Kinase Signaling System;Myocytes, Cardiac;Oxidative Stress;p38 Mitogen-Activated Protein Kinases;Phosphorylation;Protective Agents;Rats;Reactive Oxygen Species;Signal Transduction;Superoxide Dismutase
期刊:
MEDICAL SCIENCE MONITOR
ISSN:
1643-3750
年:
2018
卷:
24
页码:
2109-2118
基金类别:
The work was supported by the Hunan Provincial Natural Science Foundation of China \(2018JJ5052\), the Hunan Provincial Creation Development Project \(2013TF3006\), the National Natural Science Foundation of China \(31320103917 and 31172234\), and the Youth Innovation Team Project of ISA, CAS \(2017QNCXTD_ZCS\). Qiongxian Yan, e-mail: yanqx14@isa.ac.cn The work was supported by the Hunan Provincial Natural Science Foundation of China \(2018JJ5052\), the Hunan Provincial Creation Development Project \(2013TF3006\), the National Natural Science Foundation of China \(31320103917 and 31172234\), and the Youth Innovation Team Project of ISA, CAS \(2017QNCXTD_ZCS\)
机构署名:
本校为通讯机构
院系归属:
园艺园林学院
摘要:
Background: L-theanine is a non-protein amino acid in green tea, and its hepatoprotection and neuroprotection have been verified. However, whether L-theanine can prevent cardiomyocytes from apoptosis is unclear yet. This study evaluated the protective effects of L-theanine on H2O2-induced heart injury in vitro. Material/Methods: The certified H9C2 cells were pretreated with L-theanine (0 mM, 4 mM, 8 mM, and 16 mM) for 24 h, followed by 160 μM H2O2 solution for 4 h. The cell viability and antioxidant indices were assayed. Quantitative evaluatio...

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