期刊论文

Cloning and Sequence Analysis of Internal Transcribed Spacer 1 of Six Eimeria spp. from Chicken

中文

中国家禽

1004-6364

2009

31

13

24-27

10.16372/j.issn.1004-6364.2009.13.009

基金项目：中央级公益性科研院所基本科研业务费专项资金项目（2006JB04）,国家“十五”科技攻关计划子课题（2004BA514A15-08）

本校为第一机构

为对上海地区分离的6种鸡艾美耳球虫内转录间隔区1(ITS-1)进行克隆和序列分析,探索ITS-1区域序列在鸡球虫分类学中的作用,利用一对属特异性引物,对上海地区分离的6种球虫的ITS-1序列进行PCR扩增,扩增产物克隆到pMD18-T后进行序列测定,并与GenBank上下载的序列进行系统进化树分析.结果显示,巨型艾美耳球虫有大小分别为547bp和422bp两条PCR扩增条带;和缓艾美耳球虫有大小为627bp和493bp两条PCR扩增条带;其他4种球虫均为一条PCR扩增条带,大小分别为柔嫩艾美耳球虫668bp,毒害艾美耳球虫691 bp,堆型艾美耳球虫507 bp,早熟艾美耳球虫541 bp.6种球虫序列之间的同源性为34%～52%.系统进化分析显示,各个种...

In order to clone and analyze the sequence of internal transcribed spacer 1 （ITS-l）of Eimeria teneUa, E.necatrix,E.acervulina,E.maxima,E.praecox and E.mitis from chicken in Shanghai,a genus-specific primers was employed to amplify the ITS-I of six Eimeria species. The amplified products were cloned into pMD18-T vector and their nucleotide sequences were determined. The phylogenetic relations were analyzed by compared together with sequences from GenBank. The results showed that there were two amplified fragments for E.rnaxima and E.mitis resp...