期刊论文

中文

中国家禽

1004-6364

2019

20

38-42

10.16372/j.issn.1004-6364.2019.20.008

本校为第一机构

CRISPR/Cas9系统是新一代的基因编辑技术,由成簇重复短回文序列(Clustered regulary interspaced short palindromic repeats,CRISPR)和CRISPR相关蛋白9(CRISPR-associated protein9,Cas9)组成,具有操作成本低和高效率等优点,广泛应用于人类细胞、果蝇、斑马鱼、小鼠、猪、鸡等动物基因组的定点编辑。借助CRISPR/Cas9技术开展家禽胚胎发育、生长和屠体性状改良、繁殖性能提升等具有重要的科研和应用实践价值。文章回顾了CRISPR/Cas9系统的研究历程、分类及作用模式,重点对CRISPR/Cas9系统在家禽基因组编辑中的应用进行了综述并对发展前景做了展望,以期为家禽重要经济性状的遗传改良和分子育种探...

CRISPR/Cas9 system is a new technology for gene editing, which consists of clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated protein 9(Cas9). CRISPR/Cas9 system has showed the advantages of low operational cost and high efficiency. Up to now, CRSIPR/Cas9-mediated genome editing has been widely applied in many animals or cell lines. It has been demonstrated to be the useful tools for scientific research and practical application on investigating embryo development, improving the growth and carcass performanc...