Development and Application of a Fast Gas Chromatographic Method Offer New Insights into L-theanine Production Regulation in Camellia sinensis L.

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成果类型：

期刊论文

作者：

Chen, Yanni;Liu, Shuoqian;Ferreira, Jorge Freire da Silva;Xiao, Lizheng;Gu, Meiyi;...

通讯作者：

Zhonghua Liu<&wdkj&>Jianan Huang<&wdkj&>Na Tian

作者机构：

[Tian, Na; Luo, Yiping; Liu, Zhonghua; Liu, Shuoqian; Huang, Jianan; Chen, Yanni; Xiao, Lizheng] Hunan Agr Univ, Coll Hort, Dept Tea Sci, Changsha 410128, Peoples R China.

[Zhang, Tiantian; Gu, Meiyi; Liu, Zhonghua; Liu, Shuoqian; Huang, Jianan; Zhang, Xiangqin] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Peoples R China.

[Ferreira, Jorge Freire da Silva] USDA ARS, US Salin Lab, Riverside, CA 92507 USA.

通讯机构：

[Zhonghua Liu; Jianan Huang; Na Tian] D

Department of Tea Science, College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Department of Tea Science, College of Horticulture, Hunan Agricultural University, Changsha 410128, China

语种：

英文

关键词：

Camellia sinensis L.;l-theanine;gas chromatography;biosynthesis;quantification

期刊：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN：

0021-8561

年：

2021

卷：

期：

页码：

11142-11150

DOI：

10.1021/acs.jafc.1c04093

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [U19A2030, 32172629, 31670689]; Provincial Natural Science Foundation of HunanNatural Science Foundation of Hunan Province [2020JJ4358]; Special Project of Central Government Guiding Local Science and Technology Development [2019XF5041]; Hunan Modern Agro-industry Technology Research System [HARS-20]

机构署名：

本校为第一机构

院系归属：

园艺园林学院

摘要：

Tea is the most consumed beverage worldwide, and l-theanine in tea leaves significantly affects their flavor and market quality. We have developed and validated a fast and reliable gas chromatographic method with flame ionization detection (GC-FID) to quantify l-theanine after its extraction from Camellia sinensis (tea plant) and derivatization. The procedure was completed in 40 min, from extraction to chromatographic analysis, with a recovery rate of more than 93% and allowing a high sample throughput. The GC-FID intraday precision was within ...

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Development and Application of a Fast Gas Chromatographic Method Offer New Insights into L-theanine Production Regulation in Camellia sinensis L.

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