期刊论文

Down - regulating Cathepsin L suppresses invasion and migration of human cervical cancer cell line Hela via inhibiting EMT.

中文

广东医学

Guangdong Medical Journal

1001-9448

2014

35

22

3460-3463

广东省自然科学基金博士启动项目(编号:S2013040013849)； 广州市科技计划项目(民主科技重大专项)(编号:2012Y2-00022)； 广州医科大学校基金博士启动项目(编号:2012C54)； 南方医科大学中西医结合医院博士启动基金项目(编号:1201302009)；

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目的探讨溶酶体组织蛋白酶L(Cathepsin L)是否通过上皮-间充质转化(EMT)影响宫颈癌细胞的侵袭及迁移能力。方法利用荧光定量PCR来检测人宫颈癌细胞HeLa、CaSki以及HeLa229中Cathepsin L的表达水平;设计并合成靶向Cathepsin L的特异性shRNA,通过脂质体转染法转染Cathepsin L表达最高的宫颈癌细胞HeLa以构建稳定低表达Cathepsin L细胞株,通过Western blot和定量PCR来验证shRNA的干扰,效率,拟将Cathepsin L -shRNA和空白对照载体转染HeLa细胞,并将构建好的稳定细胞株命名为HeLa/shRNA,空白组和对照组分别为HeLa和HeLa/Con。通过Transwell法检测干扰后细胞的迁移及侵袭能力,Western blot法检测EMT相...

Objective To investigate the effect of down - regulation of Capthesin L on the invasion and migration of cervical cancer cells Hela. Methods The shRNA targeting Capthesin L was designed and synthesized, and subse- quently transfected into the Hela cells via Lipofectamine 2000 mediation. The migration ability and invasion ability of Hela cells were detected using transwell assay. Expression of E -cadherin, N -cadherin, WNT- 5a and Snail was assessed by using Western blot. Results Capthesin L - targeted shRNA could down - regulate the Capthesin L...