期刊论文

Isolation and identification of“golden flower”fungus and determination of its effects on chemical the composition of Fu tea

Zhu, M.;Fu, D.;Wang, K.

中文

辐射研究与辐射工艺学报

1000-3436

2021

39

5

96-103

10.11889/j.1000-3436.2021.rrj.39.050701

基金资助：国家重点研发计划课题（ 2018 YCF1604403 ）和国家自然科学基金（ 32002095 ）资助 第一作者：冉莉莎，女，1995 年1月出生，2018 年于遵义师范学院获得学士学位，现为湖南农业大学硕士研究生 通信作者：朱洺志，博士，硕士生导师，E-mail: mzzhucn@hotmail.com；傅冬和，博士，博士生导师，E-mail: fu7879@yahoo.com.cn；王坤波，博士，博士生导师，E-mail: wkboo163@163.com 收稿日期：初稿 2021-02-19；修回 2021-04-18 Supported by National Key Research and Development Project (2018YCF1604403) and National Natural Science Foundation of China (32002095) First author: RAN Lisha (female) was born in January 1995, and obtained her bachelors degree from Zunyi Normal College in 2018. NowsheisagraduatestudentatHunanAgriculturalUniversity Corresponding author: ZHU Mingzhi, doctoral degree, masters supervisor , E-mail: mzzhucn@hotmail.com; FU Donghe, doctoral degree, doctoral supervisor , E-mail: fu7879@yahoo.com.cn; W ANG Kunbo, doctoral degree, doctoral supervisor , E-mail: wkboo163@163.com Received19February2021;accepted18April2021

本校为第一机构

从湖南茯茶中分离得到一株“金花”菌,将其命名为ACF-1。根据ACF-1培养特征、光学显微结构特征、电子显微结构特征及ITS区序列比对分析,以及基于β-微管蛋白(β-tubulin)、钙调蛋白(Calmodulin)和RNA聚合酶Ⅱ(RNA polymerase Ⅱ)3个基因序列的多基因系统发育分析,最终将ACF-1鉴定为冠突曲霉菌(Aspergillus cristatus)。将ACF-1菌接种至灭菌黑毛茶进行茯茶散茶快速“发花”实验,分析其主要化学成分的变化情况。结果表明,与未“发花”样品相比,“发花”样品的茶多酚含量、游离氨基酸含量、可溶性糖含量和黄酮含量变化显著,分别下降11.3%、17.3%、11.9%和33.8%,说明“金花”菌参与了复杂的化学成分...

This study aimed to accurately identify the “golden flower” fungus, and characterize its effects on the chemical composition of Fu tea. A “golden flower” fungus strain was isolated from Hunan Fu tea and named as ACF-1. The ACF-1 was identified as Aspergillus cristatus using culture characteristics, optical microscopic structure, electron microscopic structure, ITS region sequence alignment analysis, and multigene phylogeny analysis based on β -tubulin, calmodulin, and RNA polymerase Ⅱ gene sequences. ACF-1 fungus was inoculated into steri...