期刊论文

Radiation mutagenesis of Bacillus velezensis and its antibacterial effect on Colletotrichum gloeosporioides

Liu, S.

中文

辐射研究与辐射工艺学报

1000-3436

2023

41

3

89-99

10.11889/j.1000-3436.2023-0029

Supported by the National Key R & D Program of China (2021YFE0111200-2) and the Agricultural Science and Technology Innovation Project of Hunan Province (2022CX119)

本校为第一机构

采用60Co-γ射线和电子束辐射诱变贝莱斯芽孢杆菌选育遗传稳定的突变菌株,用平板对峙法及四点法对突变菌株进行初筛和复筛,研究其对辣椒炭疽病菌的抑菌效果。结果表明:菌悬液经60C...展开更多 采用60Co-γ射线和电子束辐射诱变贝莱斯芽孢杆菌选育遗传稳定的突变菌株,用平板对峙法及四点法对突变菌株进行初筛和复筛,研究其对辣椒炭疽病菌的抑菌效果。结果表明:菌悬液经60Co-γ射线辐照,贝莱斯芽孢杆菌D10值为2366 Gy,在100~2000 Gy诱变剂量范围内芽孢杆菌致死率随着辐照吸收剂量的增加而升高。在2000 Gy辐照吸收剂量下,贝莱斯芽孢杆菌的致死率为81.8%,并筛选出5株(B004、B112、B114、B117、B118)...

We used60Co-γray and electron beam irradiation to mutate Bacillus velezensis to breed genetically stable mutant strains.The mutant strains were screened and re-screened by plate confrontation and the four-point method to study their antibacterial effects on C...MORE We used60Co-γray and electron beam irradiation to mutate Bacillus velezensis to breed genetically stable mutant strains.The mutant strains were screened and re-screened by plate confrontation and the four-point method to study their antibacterial effects on Colletotrichum gloeospo...