期刊论文

Fang, J

英文

Molecular Biotechnology

1073-6085

2024

1-13

10.1007/s12033-024-01098-6

This study was supported by Hunan Provincial Science and Technology Department (2021JJ30008, 2020NK2004, 2019TP2004, 2017NK2322, 2016WK2008, 2016TP2005), Double first-class construction project of Hunan Agricultural University (SYL201802003), Postgraduate Scientific Research Innovation Project of Hunan Province (CX20210654) and Science and Technology Innovation and Entrepreneurship Project for University Students of Hunan Province (2021RC1004).

本校为第一且通讯机构

Phycocyanobilin (PCB) is a blue pigment with antioxidant, anti-inflammatory, and anticancer properties. It is used in the medical and cosmetic industries. In this study, a high-expression plasmid, pET-30a-PCB, was constructed for expression of PCB in Escherichia coli BL21(DE3). The PCB was analyzed using UV-visible absorption spectrum, MALDI-TOF-MS, and fluorescence spectra. The stability and half-life of PCB in different serum were determined. The yield of PCB was optimized through single-factor and orthogonal experiments. The optimal expression conditions were determined as a lactose concent...