The aim of the present investigation was to determine the active ingredients in Amaran-thus tricolor L. leaves and develop a biological pesticide. Organic solvent extraction, column chro-matography, liquid chromatography, ODS-C18 reverse elution, Sephadex LH-20 gel filtration, H spectrum, and C spectrum were used to isolate the pure product for an assessment of the agricultural activity and bacteriostatic mechanisms. The results showed that the activity of the crude extract following carbon powder filtration was 1.63-fold that of the non-filter...
