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Simple and sensitive detection of acrylamide based on hemoglobin immobilization in carbon ionic liquid paste electrode

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成果类型:
期刊论文
作者:
Li, Na;Liu, Xiaoying;Zhu, Jihe;Zhou, Bijing;Jing, Junxian;Wang, Aibing;Xu, Rui;Wen, Zhou;Shi, Xingbo;Guo, Shiyin*
通讯作者:
Guo, Shiyin
作者机构:
[Shi, Xingbo; Li, Na; Zhu, Jihe; Guo, Shiyin] Hunan Agr Univ HUNAU, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
[Liu, Xiaoying; Xu, Rui; Jing, Junxian; Zhou, Bijing] Hunan Agr Univ HUNAU, Coll Sci, Changsha 410128, Hunan, Peoples R China.
[Wang, Aibing] Hunan Agr Univ HUNAU, Coll Vet Med, Key Lab Anim Vaccine & Prot Engn, Lab Anim Models & Funct Genom, Changsha 410128, Hunan, Peoples R China.
[Wen, Zhou] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Waihuan Rd, Guangzhou 510006, Guangdong, Peoples R China.
通讯机构:
[Guo, Shiyin] Hunan Agr Univ HUNAU, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Acrylamide;Hemoglobin;Carbon ionic liquid paste electrode;Electrochemical biosensor;Detection
期刊:
Food Control
ISSN:
0956-7135
年:
2019
卷:
109
期:
Volume 109
页码:
106764-
文献类别:
WOS:Article
所属学科:
WOS学科类别:Food Science & Technology
入藏号:
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21606081, 21605077]; Introduce Talents Fund of Hunan Agricultural University [13YJ01]; General Project of Hunan Education Department [18C0174]
机构署名:
本校为第一且通讯机构
院系归属:
动物医学院
食品科学技术学院
理学院
摘要:
A simple and sensitive electrochemical biosensor for acrylamide (AA) detection based on hemoglobin (Hb) entrapped in ionic liquid-carbon paste was constructed. The Hb-carbon ionic liquid paste electrode (Hb/CILPE) was studied and characterized by scanning electron microscopy (SEM) and electrochemical method. The surface passivation of the Hb/CILPE could be achieved by the addition of acrylamide molecule, which caused the lower current response value owing to the adduct formed by acrylamide and Hb as a result of the reaction between the a-NH2 group and the N-terminal valine of Hb. The experimental conditions such as pH value of supporting electrolyte, pulse increment and concentrated time of AA was examined. Under the optimum conditions, the differential pulse voltammetry (DPV) responses of Hb/CILPE toward AA was evaluated in a good linear range from 1.0 x 10(-11) to 1.0 x 10(-5)M, with a detection limit of 5.0 x 10(-12)M at S/N = 3. This biosensor can be successfully applied to the determination of AA in a real sample without complex pretreatment. Compared with other electrochemical methods for detecting AA, the prepared electrochemical biosensor exhibited advantages of simple preparation, easy surface renewal, better reproducibility and satisfactory stability.
参考文献:
Alahari A, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0001343
Baghbamidi SE, 2016, INT J ELECTROCHEM SC, V11, P10874, DOI 10.20964/2016.12.92
Batra B, 2013, BIOPROC BIOSYST ENG, V36, P1591, DOI 10.1007/s00449-013-0931-5
Batra B, 2013, ANAL BIOCHEM, V433, P210, DOI 10.1016/j.ab.2012.10.026
Bedogni G, 2008, AM J GASTROENTEROL, V103, P2248, DOI 10.1111/j.1572-0241.2008.01948.x

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