Nigrospora oryzae is a pathogen that can infect plants of various species. Here, we report the isolation of a novel mycovirus from N. oryzae infecting rice, as well as the complete genome sequence and genomic organization of this virus, which we have named “Nigrospora oryzae nonsegmented RNA virus 1” (NoNRV1). The genome of NoNRV1 contained two non-overlapping open reading frames (ORF1 and ORF2) potentially encoding a protein with an unknown function in ORF1 and a putative RNA-dependent RNA polymerase (RdRp) in ORF2. Homology and phylogenetic...