期刊论文

Zhang, X.-W.

中文

湖南师范大学自然科学学报

2096-5281

2001

24

1

62-65, 77

10.3969/j.issn.1000-2537.2001.01.017

国家教育部留学基金资助项目!（教外司留 [2 0 0 0 ]36 7号 ）；

本校为第一机构

A21是拟南芥的一同源转换盒基因,在发育旺盛的组织中高度表达。采用反义RNA技术,将该基因cDNA 5'端片段410bp反向克隆到pTA7002后,构建成了可在植物中调控表达A21反义RNA基因片段的重组体。以根癌农杆菌浸渍法将重组体转入拟南芥Landsberg erecta生态型植株中,筛选到转A21反义RNA基因的拟南芥植株。反义基因可在甾类激素诱导下表达出A21的反义RNA。经初步诱导表达,变异性状表现为顶端生长抑制型。

A21 is a homeobox gene of Arabidopsis thaliana and it is expressed in all active developing tissues. A 5′fragment of 410bp of A21 cDNA is cloned into a T-DNA vector pTA 7002 in a reverted direction under the control of a regulated promoter. Thus an antisense A21 gene is constructed. The antisense recombinant is transformed into Arabidopsis thaliana ecotype of Landsberg erecta via infiltration. Transgenic plants are selected out of hygromycin resistance. The antisense RNA can be transcripted by glucocorticoid in...