With 18srDNA as as endogenous reference, relative quantification detection of Cucumber Mosaic Virus (CMV) in Pinellia Ternata and its tissue cultured plantlets was carried by using Real-time PCR, and combined with methods of DAS-ELISA and RT-PCR. The results showed that CMV concentration in tissue cultured plantlets was decreased greatly compared with the parent plantlet. CMV quantity in leaf of tissue cultured plantlets which come from explant of CMV positive Pinellia Ternata was relatively higher than that of tubercles and callus. There were fake positive results while using ELISA method to ...