期刊论文

Lianfei Tang

英文

中国免疫学杂志：英文版

1672-7681

2007

4

2

153-156

Acknowledgements This work received financial support from the UNDP/World Bank/WH0 Special Program for Research and Training in Tropical Diseases （TDR） contract 980268 and from the 0ffice for Schistosomiasis Control of Hunan Health Bureau of the People＇s Republic of China. We thank Shunke Zhang, Ganwei Yan, Xinshong Luo, Zhongjie Li and Qinren Zeng for their excellent technical assistance and Xiaoye Wu for her precious help. We thank our colleagues at Department of Parasitology for their support.

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Two Schistosoma japonicum vaccine candidate antigens Sj 31 and Sj 32, which have shown particular promise to induce protective immunity in mice, were used to immunize goats by using a DNA priming-protein boosting strategy in present work. DNA vaccine formulations of the two antigens (VRSj31 and VRSj32) were produced and injected intramuscularly twice at a 2-week interval and then recombinant proteins (rSj31 and rSj32) together with Freund Complete Adjuvant (FCA) were used to boost the goats. The experiment was repeated in different batche cercariae. A strong anamnestic antibody response was in...

二血吸虫 japonicum 疫苗候选人抗原 Sj 31 和 Sj 32，显示了特别诺言在老鼠导致保护的免疫，被用来整洁蛋白质的增加由使用 DNA 使山羊免疫在现在的工作的策略。二抗原(VRSj31 和 VRSj32 ) 的 DNA 疫苗的明确的表达和 Freund 完全的助手(农业信贷) 在 2 星期的间隔然后 recombinant 蛋白质(rSj31 和 rSj32 ) 肌内地被生产并且注射两次被用来增加山羊。实验在不同 batche cercariae 被重复。强壮的抗体再生的抗体回答在增加以后被导致。肝鸡蛋计数并且 miracidial 孵化的重要减小在两个实验被显示出。对挑战感染的重要保护在第二个实验为蠕虫恢复与 31.6% 百分比减小被得到并且 20.9% 在第一个实...