Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of a Bymovirus P3N-PIPO Gene Expressed from a PVX Vector in Nicotiana benthamiana

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Yu, Chulang;Miao, Runpu;Ye, Zhuangxin;MacFarlane, Stuart;Lu, Yuwen;...

作者机构：

[Yu, Chulang; Dai, Liangying; Chen, Jianping] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Peoples R China.

[Miao, Runpu; Lu, Yuwen; Li, Junmin; Yang, Jian; Yan, Fei; Yu, Chulang; Chen, Jianping; Ye, Zhuangxin] Ningbo Univ, Inst Plant Virol, Minist Agr & Zhejiang Prov,Key Lab Biotechnol Pla, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China.

[MacFarlane, Stuart] James Hutton Inst, Cell & Mol Sci, Dundee DD2 5DA, Scotland.

语种：

英文

关键词：

P3N-PIPO;transcriptional slippage;PVX;Bymovirus

期刊：

Viruses

ISSN：

1999-4915

年：

2021

卷：

期：

页码：

1247

DOI：

10.3390/v13071247

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31801703]; K.C. Wong Magna Fund in Ningbo University

机构署名：

本校为第一机构

院系归属：

植物保护学院

摘要：

P3N-PIPO (P3 N-terminal fused with Pretty Interesting Potyviridae ORF), the movement protein of potyviruses, is expressed as a translational fusion with the N-terminus of P3 in potyviruses. As reported in previous studies, P3N-PIPO is expressed via transcriptional slippage at a conserved G2A6 slippery site in the genus Potyvirus. However, it is still unknown whether a similar expression mechanism of P3N-PIPO is used in the other genera of the family Potyviridae. Moreover, due to the extremely low expression level of P3N-PIPO in natural virus-infected plants, the peptides spanning the slippery ...

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Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of a Bymovirus P3N-PIPO Gene Expressed from a PVX Vector in Nicotiana benthamiana

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