Analysis of genetic diversity and development of Kompetitive allele specific PCR (KASP) markers for Lithocarpus litseifolius (Hance) Chun resources based on dd-RAD-Seq

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成果类型：

期刊论文

作者：

Tian, Yuqiao;Xiao, Shengen;Chen, Yangfeng;Li, Ziqiang;Wang, Minglei;...

通讯作者：

Qiu, XY

作者机构：

[Wu, Xianjin; Tian, Yuqiao; Qiu, Xiaoyan] HuaiHua Univ, Coll Biol & Food Engn, Huaihua 418000, Peoples R China.

[Xiao, Shengen; Li, Ziqiang; Wang, Minglei; Tian, Yuqiao; Chen, Yangfeng] Hunan Agr Univ, Coll Hort, Changsha 410000, Peoples R China.

[Xiao, Shengen; Wu, Xianjin; Xiang, Zhuwen; Li, Ziqiang; Wang, Minglei; Wang, Yiqing; Qiu, Xiaoyan; Chen, Yangfeng] Hunan Yao Tea Engn Technol Res Ctr, Xupu 419300, Peoples R China.

[Li, Jin] Shaoyang Acad Agr Sci, Inst Tea Res, Shaoyang 422000, Peoples R China.

通讯机构：

[Qiu, XY ] H

HuaiHua Univ, Coll Biol & Food Engn, Huaihua 418000, Peoples R China.

Hunan Yao Tea Engn Technol Res Ctr, Xupu 419300, Peoples R China.

语种：

英文

关键词：

Lithocarpus litseifolius (Hance) Chun;Single nucleotide polymorphism;KASP;DNA fingerprinting;Germplasm identification

期刊：

Genetic Resources and Crop Evolution

ISSN：

0925-9864

年：

2025

页码：

1-12

DOI：

10.1007/s10722-025-02462-4

基金类别：

This investigation was financially supported by several funding sources, including the Outstanding Youth Project of the Hunan Provincial Department of Education [24B0710], the Natural Science Foundation of Hunan Province [2332], the Key Project of Huaihua titled “Study on selection of excellent germplasm resources and key techniques of deep processing of Xupu Yao tea” [2022], the Furong Plan project “Key Technology Research and Development of L. litseifolius and Industrialization Innovation and Entrepreneurship Team” [2022], and the Central Government’s Guidances Fund for Local Science and Technology Development [2024ZYQ131].

机构署名：

本校为其他机构

院系归属：

园艺园林学院

摘要：

Lithocarpus litseifolius (Hance) Chun, known as “sweet tea”, is a Fagaceae family plant with significant commercial and medicinal value. This study evaluated the genetic diversity and created DNA fingerprints of 38 L. litseifolius germplasm resources collected from 12 provinces across China. Double-digest restriction-site associated DNA sequencing (dd-RAD-seq) was used to develop single nucleotide polymorphism (SNP) markers. A total of 4864 SNPs were selected for characterizing the population genetic analysis. Phylogenetic analysis grouped th...

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Analysis of genetic diversity and development of Kompetitive allele specific PCR (KASP) markers for Lithocarpus litseifolius (Hance) Chun resources based on dd-RAD-Seq

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