期刊论文

Identification of Brown Planthopper Resistance Genes in Broad-spectrum Blast Resistant Rice Germplasm 75-1-127 and Its Molecular Marker-Assisted Selection Breeding

Xiao, Y.

中文

中国水稻科学

1001-7216

2019

33

3

227-234

10.16819/j.1001-7216.2019.9005

国家重点研发计划资助项目(2016YFD0101100) 湖南省科技重大专项(2015NK1001-1) 湖南省研究生科研创新项目(CX2015B255)。

本校为第一且通讯机构

【目的】水稻品系75-1-127携带广谱抗稻瘟病基因Pi9,已被广泛应用于抗稻瘟病水稻品种改良。笔者育种实践发现75-1-127表现出较强的褐飞虱抗性,因此鉴定该品系中的褐飞虱抗性基因并进行分子辅助选择育种。【方法】根据水稻品系B5中褐飞虱抗性基因Bph14和Bph15的序列,设计引物扩增75-1-127的基因组DNA,并对PCR产物进行测序分析。采用苗期集团法鉴定了75-1-127和B5的褐飞虱抗性表型。利用与Bph14与Bph15连锁的分子标记筛查了75-1-127为稻瘟病抗源回交转育的两系不育系后代,并鉴定了这些后代的稻瘟病抗性、褐飞虱抗性和主要农艺性状。【结果】75-1-127中含有与B5完全一致的Bph14和Bph15序列。75-1-1...

(Objective) Rice line, 75-1-127, which harbors the broad-spectrum blast resistance gene Pi9, has been widely used in rice variety improvement for blast resistance. Given that 75-1-127 shows strong resistance to brown planthoppers in our previous breeding projects, we identified its resistance genes to brown planthoppers and used it in molecular marker-assisted selection breeding. (Method) The PCR products, which amplified from the genomic DNA of 75-1-127 with the primers designed according to the sequence of Bph14 and Bph15 genes in rice line B...