Establishment and application of multiplex real-time PCR for simultaneous detection of four viruses associated with porcine reproductive failure

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Chen, Yuan;Luo, Shile;Tan, Jianmei;Zhang, Luhua;Qiu, Shengwu;...

通讯作者：

Wang, C.;Zhan, Y.

作者机构：

[Wang, Aibing; Chen, Yuan; Qiu, Shengwu; Zhang, Luhua; Wang, Naidong; Zhan, Yang; Luo, Shile; Deng, Zhibang; Tan, Jianmei; Yang, Qing; Yang, Yi] Hunan Agr Univ, Coll Vet Med, Res Ctr Reverse Vaccinol RCRV, Prov Key Lab Prot Engn Anim Vaccines, Changsha, Hunan, Peoples R China.

[Wang, Aibing; Chen, Yuan; Qiu, Shengwu; Zhang, Luhua; Wang, Naidong; Zhan, Yang; Luo, Shile; Deng, Zhibang; Tan, Jianmei; Yang, Qing; Yang, Yi] Hunan Agr Univ, Coll Vet Med, Lab Funct Prote LFP, Changsha, Hunan, Peoples R China.

[Hao, Zhiyou] Anim Dis Prevent & Control Ctr Yongzhou, Yongzhou, Hunan, Peoples R China.

[Wang, Changjian] Anim Dis Prevent & Control Ctr Hunan Prov, Changsha, Hunan, Peoples R China.

通讯机构：

[Zhan, Y.] P

[Wang, C.] A

Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan, China

Animal Disease Prevention and Control Center of Hunan Province, Hunan, China

语种：

英文

关键词：

Porcine circovirus type 2 (PCV2);porcine circovirus type 3 (PCV3);Porcine parvovirus (PPV);Pseudorabies virus (PRV);Multiplex real-time PCR

期刊：

Frontiers in Microbiology

ISSN：

1664-302X

年：

2023

卷：

页码：

1092273

DOI：

10.3389/fmicb.2023.1092273

基金类别：

This work was supported by National Natural Science Foundation of China (32172844 and 32202790), Hunan Provincial Natural Science Foundation of China (2022JJ30298 and 2022JJ40183), Natural Science Foundation of Changsha City (kq2202231), the Young Talents Science and Technology Project in Hunan Province (2022RC1046), and Hunan Province Technology Breakthrough Project of 2021 for the open competition mechanism to select the best candidates (grant number: 2021NK1030).

机构署名：

本校为第一机构

院系归属：

动物医学院

摘要：

Many pathogens cause reproductive failure in sows suffering a broad spectrum of sequelae, including abortions, stillbirth, mummification, embryonic death, and infertility. Although various detection methods, such as polymerase chain reaction (PCR) and real-time PCR, have been widely used for molecular diagnosis, mainly for a single pathogen. In this study, we developed a multiplex real-time PCR method for the simultaneous detection of porcine circovirus type 2 (PCV2), porcine circovirus type 3 (PCV3), porcine parvovirus (PPV) and pseudorabies v...

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Establishment and application of multiplex real-time PCR for simultaneous detection of four viruses associated with porcine reproductive failure

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