Aromatase, encoded by Cyp19a1, is essential in estrogen biosynthesis and crucial in sex differentiation and ovary development. Although aromatase regulates the proliferation, development, maturation and functional maintenance of male germ cells during mammalian spermatogenesis, its role in nonmammalian vertebrates is unclear. In the current study, a polyclonal antibody was prepared and the function of aromatase in testes was determined by administering letrozole. With ELISA detection, the titer of the antibody prepared in this study was more than 1:30000. Western blot analysis showed that the ...