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Characterization of aromatase in testes and potential functional analysis with letrozole administration in male Chinese soft-shelled turtle (Pelodiscus sinensis)

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成果类型:
期刊论文
作者:
Ma, Xiao;Wang, Guiyu;Liu, Huifen;Wu, Limin;Tian, Xue;...
通讯作者:
Xiaoqing Wang<&wdkj&>Xuejun Li
作者机构:
[Zhang, Chao; Wang, Luming; Li, Xuejun; Wang, Guiyu; Liu, Huifen; Wu, Limin; Feng, Junchang; Cen, Shuangshuang; Ma, Xiao; Tian, Xue] Henan Normal Univ, Fisheries Coll, Xinxiang 453007, Henan, Peoples R China.
[Wang, Luming; Wang, Xiaoqing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.
[Wu, Qisheng] Fisheries Res Inst Fujian, Key Lab Cultivat & High Value Utilizat Marine Org, Xiamen 361000, Peoples R China.
通讯机构:
[Xiaoqing Wang] C
[Xuejun Li] F
Fisheries College, Henan Normal University, Xinxiang 453007, China<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
语种:
英文
关键词:
Aromatase;Letrozole;Pelodiscus sinensis;Polyclonal antibody;Spermatogenesis
期刊:
Aquaculture
ISSN:
0044-8486
年:
2021
卷:
544
页码:
737089
基金类别:
This work was supported by grants from the National Natural Science Foundation of China (No. 31702316 and 31902371 ), National Key R&D Program of China ( 2018YFD0900200 ), Key Scientific and Technological Project of Henan Province ( 212102110384 ), Open Program of Key Laboratory of Cultivation, High-value Utilization of Marine Organisms in Fujian Province ( 2018fjscq05 ) and Key Research Programs for Institutions of Higher Education in Henan Province ( 15A240002 and 17A240002 ).
机构署名:
本校为其他机构
院系归属:
动物科学技术学院
摘要:
Aromatase, encoded by Cyp19a1, is essential in estrogen biosynthesis and crucial in sex differentiation and ovary development. Although aromatase regulates the proliferation, development, maturation and functional maintenance of male germ cells during mammalian spermatogenesis, its role in nonmammalian vertebrates is unclear. In the current study, a polyclonal antibody was prepared and the function of aromatase in testes was determined by administering letrozole. With ELISA detection, the titer of the antibody prepared in this study was more than 1:30000. Western blot analysis showed that the ...

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