Knowledge acquisition is the bottleneck in developing expert system. It usually takes a long period to acquire disease knowledge using the traditional methods. Aiming at this problem, the paper presents the relationship between rough sets and rule-based rapeseed disease knowledge, namely the application of rough sets in knowledge acquisition from rapeseed disease expert. Then the exclusive rules, inclusive rules and disease images of rapeseed disease are built based on the RHINOS diagnosis model, and the definition of probability rule is put forward. At last, the paper presents the rule-based ...