期刊论文

中文

南方

1006-1304

2007

15

3

459-463

10.3969/j.issn.1674-7968.2007.03.018

湖南省教育厅项目（No.04C286）资助.

本校为第一机构

应用mRNA差异显示技术（DDRT-PCR）,对瓜叶菊（Senecio×hybridus）离体筛选获得的耐热变异体N1-2-1及其母株N1在热胁迫下的基因差异表达进行分析。结果显示,从40对引物中共扩增出1419条,筛选到100条在热胁迫下耐热变异体N1-2-1和母株N1间差异明显的基因片段,差异率为7.05%,其中41条差异片段来自N_(1-2-1),其中有6个片段（DF1～DF6）经过Northern杂交验证只在N_(1-2-1)中表达而在母株N1中不表达。进一步克隆测序和数据库比对分析表明,DF3和DF4分别与马铃薯和水稻热胁迫时产生的应答反应有关,其中DF4与热休克蛋白HSP70部分序列高度同源,DF3与HSP结合蛋白部分序列高度同源;DF1与花色素苷转酰基酶有...

Gene expression in heat-tolerant variants N1-2-1 and its mother plants N1 under heat stress were studied by differential display reverse transcript polymerase chain reaction （DDRT-PCR）. The results showed that 1 419 bands were amplified from 40 pairs of primers in N1-2-1 and N1, in which 100 of them appeared different obviously between them, which accounted for 7.05%. In these different bands, 41 of them derived from heat-tolerant variants N1-2-1. The Northem blotting results showed that 6 segments （DF1～DF6） were confirmed to express only ...