期刊论文

Recombination Porcine Pm MetQ Induced Immune Protective Efficacy Against Challenged Infection of Pasteurella multocidain in Mice (Mus musculus)

中文

农业生物技术学报

1674-7968

2017

25

10

1682-1688

10.3969/j.issn.1674-7968.2017.10.014

基金项目：湖南省自然科学基金项目（No.14JJ4077）

本校为第一机构

猪多杀性巴氏杆菌(Pasteurella multocidain, Pm)感染可引起猪(Sus scrofa)产生猪肺疫、猪出血性败血症和猪萎缩性鼻炎等。目前应用的灭活全菌苗交叉保护效果差且免疫保护期短,重组疫苗尚不能提供高的保护率。为研究猪多杀性巴氏杆菌重组甲硫氨酸转运体Q(recombinant methionine transporter Q, rMetQ)的免疫保护性,本研究根据GenBank中登录的Pm metQ基因序列,根据去除影响表达的长片段疏水区的相应核苷酸序列设计引物对,以Pm的基因组为模板PCR扩增metQ基因,测序进行生物信息学分析。结果表明,猪Pm metQ基因片段含768 bp核苷酸,编码255个氨基酸的蛋白质,其理论分子量为28.14 kD,是与细菌的毒性有...

Porcine (Sus scrofa) Pasteurella multocidain (Pm) infection caused serious diseases including swine plague, hemorrhagic septicaemia and infectious atrophic rhinitis. The present inactivated vaccine had poor cross protection effect and short immune protection period, and the recombinant vaccine still did not provide high protection rate. Based on Pm methionine transporter Q (metQ) sequences in GenBank, the primers were designed after removing the corresponding nucleotide sequence of expressing hydrophobic amino acid regions to express metQ in Es...