期刊论文

Immune Protection Research Induced by Actinobacillus pleuropneumoniae Recombinant NLPI Protein Against Challenge Infection in Mice (Mus musculus)

中文

农业生物技术学报

1674-7968

2018

26

8

1392-1400

10.3969/j.issn.1674-7968.2018.08.012

湖南省教育厅重点课题（No.16A101）；

本校为第一机构

猪传染性胸膜肺炎 （porcine contagious pleuropneumonia, PCP）是猪（Sus scrofa）的一种呼吸道疾病，影响世界养猪业，其病原菌为猪胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae, APP），现有疫苗由于保护效果不甚理想，因此有必要寻找新的疫苗候选分子。本研究检测了猪APP重组NLPI蛋白（recombinant NLPI, rNLPI）的免疫保护作用。根据GenBank中已报道的APP nlpi基因序列设计引物对，然后以 APP的基因组为模板PCR 扩增nlpi基因，测序后进行生物信息学分析，发现该基因（GenBank No.： MH027649）由900 bp组成，编码1个含299个氨基酸的蛋白质，为一外膜脂蛋白，此外膜蛋白在多种APP血...

Porcine contagious pleuropneumonia (PCP) is a serious respiratory disease with great harm to pigs (Sus scrofa), caused by Actinobacillus pleuropneumoniae (APP) infection. It is one of the major epidemic diseases in the world pig industry. Existing vaccines can not give full protection against App infection, so it is necessary to screening new vaccines. In this study, immune protection of recombinant NLPI (rNLPI) vaccination against App challenged infection was detected. Based on APP nlpi sequences in GenBank, the primers were designed. APP nlpi...