期刊论文

Cloning,Structure and Expression Analysis of ADSL Gene in Xupu Goose (Anser cygnoides)

中文

农业生物技术学报

1674-7968

2019

27

2

289-296

10.3969/j.issn.1674-7968.2019.02.011

湖南省重点研发计划—农业领域技术创新项目(No.2016NK2106).

本校为第一机构

腺苷琥珀酸裂解酶（adenylosuccinate lyase, ADSL）属于天冬氨酸酶/延胡索酸酶超级家族成员,在嘌呤核苷酸起始合成和嘌呤核苷酸循环中发挥重要作用,同时对改善肌肉风味具有重要意义。为研究溆浦鹅（Anser cygnoides）ADSL在不同组织中的表达规律,本研究以溆浦鹅为实验对象,利用cDNA末端快速扩增（rapid amplification of cDNA ends, RACE）技术对溆浦鹅ADSL基因序列进行克隆,并对其核酸序列及蛋白进行生物信息学分析,同时利用qRT-PCR技术检测ADSL基因在80日龄溆浦鹅不同组织中的表达量。结果显示,克隆获得的溆浦鹅ADSL基因序列（GenBank No. MK161097）全长为1 496 bp,其中5’UTR为202 bp,3’U...

The adenylsuccinate lyase(ADSL) belongs to a member of the aspartase/fumarate superfamily family, and plays an important role in the initial synthesis of purine nucleotides and purine nucleotide cycle as well as improving muscle flavor. This study was to clone the cDNA full length of ADSL gene(GenBank No.MK161097) of Xupu goose(Anser cygnoides) by rapid amplification of cDNA ends(RACE), and analyze the ADSL mRNA level in different tissues/organs at sexual age(80 d), the nucleic acid and protein sequences by ADSL conducted by bioinformatics anal...