期刊论文

大丽轮枝孢(Verticillium dahliae) Asp f2类蛋白(VDAL)在水稻(Oryza sativa)中抵御真菌病害及提高产量的功能分析

中文

农业生物技术学报

1674-7968

2020

28

12

2091-2107

10.3969/j.issn.1674-7968.2020.12.001

国家重点研发项目（2018YFD0200502）；

本校为其他机构

病原菌分泌的多肽可作为植物防御反应的激发因子。大丽轮枝孢（Verticillium dahliae） Asp f2类蛋白（VDAL）为大丽轮枝孢（俗称黄萎菌）的外泌蛋白,属于烟曲霉（Aspergillus fumigatus） f2类锌离子结合蛋白。通过工程菌发酵等工艺将棉花（Gossypium spp.）黄萎菌Vd146菌系VDAL146蛋白制成干粉,其稀释液喷施于叶面,能够激活水稻（Oryza sativa）等植物的免疫反应,并促进植物生长发育。本研究对VDAL146 （MT974503）进行分离和鉴定,在水稻破口期前后两次叶面喷施VDAL146。结果显示,水稻对纹枯病的抗性显著提高,穗粒数和千粒重增加,最终增产14.5%;同时,VDAL146没有显示直接的抑菌活性。另外,转录...

Peptides secreted by pathogens serve as elicitors of plant defense responses. Verticillium dahliae Asp f2-like protein (VDAL) is secretory protein of V. dahliae and belongs to Aspergillus fumigatus f2-like zinc-binding protein. The VDAL146 protein in V. dahliae Vd146 strain of cotton (Gossypium spp.) was made into dry powder by engineering bacteria fermentation, and its diluent was sprayed on the leaves, which could activate the immune response of plants such as rice (Oryza sativa) and promote the growth and development of plants. In present st...