期刊论文

中文

中国病毒学：英文版

1674-0769

2002

17

2

178-181

国家自然科学基金资助项目 （ 30 170 730 ）； 国家“86 3”高科技攻关项目 （ 10 1 0 5 0 5 0 4）； 淡水生态与生物技术国家重点实验室开放基金 （ 2 0 0 2FB0 5 ）；

本校为其他机构

从湖南长沙分离到一株致病性强的草鱼呼肠孤病毒(GCRV991),该病毒能使草鱼CIK,肥头鲤FHM细胞产生明显的CPE,对水生动物BF2,EPC及哺乳动物BHK,VERO细胞株不敏感.中和实验显示,GCRV873抗体能有效地中和GCRV991病毒颗粒,形成抗原抗体免疫复合物.纯化的病毒核酸与蛋白经SDS-PAGE分离,分别呈现11条清晰的核酸带及5条主要与2条微量结构多肽图谱,其核酸蛋白分子量大小与GCRV873相近似.该毒株基因组总分子量为14.48×106kD,大小范围是0.55～2.61×106kD;5条主要与两条微量结构多肽分子量近似值分别为136kD、132kD、65kD、43kD、34kD及138kD、82kD.上述结果提示,新分离的GCRV991与GCRV873具有相似的抗原性...

A powerful pathogenic Grass Carp reovirus (GCRV\-\{991\}) was recently isolatered from Changsha, Hunan provice of China. The virus replicated well in CIK,FHM cell line, but did not infect another 2 aquatic BF2, EPC and 2 mammalian BHK, Vero cell lines. It showed that the virus could be neutrialized by GCRV\-\{873\} antibody, and also the immunocompound was detected by electronic microscope. polyacrylamide gel electrophoesis revealed that GCRV\-\{991\} genome was composed of 11 segments of dsRNA, and its structural protein was consisted of 5 maj...