期刊论文

Hu, Zhihong

英文

中国病毒学：英文版

1674-0769

2017

32

2

147-154

10.1007/s12250-016-3929-8

supported partly by the National Natural Science Foundation of China (No. 31371995 and 31621061) the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB11030400) STS Project of the Chinese Academy of Sciences (grant KFJ-SW-STS143-3) the Hunan Provincial Natural Science Foundation for Distinguished Young Scholar of China (14JJ1023)

本校为其他机构

No ascovirus isolated from China has been sequenced so far. Therefore, in this study, we aimed to sequence the genome of Heliothis virescens ascovirus 3h (HvAV-3h) using the 454 pyrosequencing technology. The genome was found to be 190,519-bp long with a G+C content of 45.5%. We also found that it encodes 185 hypothetical open reading frames (ORFs) along with at least 50 amino acids, including 181 ORFs found in other ascoviruses and 4 unique ORFs. Gene-parity plots and phylogenetic analysis revealed a close relationship between HvAV-3h and three other HvAV-3a strains and a distant relationship...

从中国孤立的 ascovirus 到目前为止都没被定序。在这研究，因此，我们试图用 454 pyrosequencing 技术定序 Heliothis virescens ascovirus 3h (HvAV-3h ) 的染色体。染色体与 45.5% 的一个 G+C 内容被发现是 190,519-bp 长。我们也发现它与至少 50 氨基酸一起编码 185 个假想开的读物框架(ORF ) ，包括在另外的 ascoviruses 发现的 181 ORF 和 4 唯一的 ORF。基因同等值阴谋和种系发生的分析揭示了在 HvAV-3h 和三另外的 HvAV-3a 紧张之间的一种靠近的关系和与 Spodoptera frugiperda ascovirus 的一种远关系 1a (SfAV-1a ) ， Trichoplusia ni ascovirus 6a (TnAV-6a ) ，和 Diadromus pulch...