期刊论文

中文

中成药

1001-1528

2012

34

11

2068-2072

10.3969/j.issn.1001-1528.2012.11.005

本校为第一机构

目的探讨灵芝多糖(Ganoderma lucidum polysaccharide)联合5-氟尿嘧啶对人结肠癌LoVo细胞体外增殖及凋亡的影响。方法采用MTT法测定单用灵芝多糖、5-氟尿嘧啶和两药合用对人结肠癌LoVo细胞的协同抑制作用,应用联合指数法分析两药物之间相互作用,流式细胞术(flow cytometry,FCM)检测灵芝多糖联合5-氟尿嘧啶对LoVo细胞周期的和凋亡抑制率的变化。结果MTT结果显示灵芝多糖和5-氟尿嘧啶单独用药均能显著抑制LoVo细胞增殖。低质量浓度灵芝多糖(< 14.70 mg/mL)和5-氟尿嘧啶(

AIM To investigate the combined use of Ganoderma lucidum polysacchrides and 5-fluorouracil on the proliferation and apoptosis of human colon carcinoma LoVo cells in vitro.METHODS The synergistic inhibiting effect of Ganoderma lucidum polysacchrides and 5-fluorouracil used alone or jointly on the proliferation of LoVo cells was measured by using MTT assay.The combined index method was used to analyze their inhibiting effect.The influence of Ganoderma lucidum polysacchrides and 5-fluorouracil combined on cell cycle and apoptosis rate of LoVo cell...