期刊论文

中文

中成药

1001-1528

2013

35

3

580-584

10.3969/j.issn.1001-1528.2013.03.036

31100502:国家自然科学基金 20114320120004:高等学校博士学科点专项科研基金

本校为其他机构

目的 测定不同地区香薷各部位总黄酮和游离黄酮的含有量,找出含黄酮量较高的药材产地和黄酮的主要储存部位.方法 以紫外可见分光光度法(UV-Vis)测定香薷总黄酮,最适条件是0.05 mol/L三氯化铝,pH值为5.0的乙酸缓冲液,水浴温度50℃,水浴时间30 min,检测波长420 nm.采用反相高效液相色谱法测定3种主要游离黄酮,色谱柱为Welchrom TM C18(200 mm×4.6 mm,5μm),以0.4％磷酸水溶液(A)-甲醇(B)为流动相,梯度洗脱；体积流量1.0 mL/min；检测波长350 nm；柱温25℃.结果 木犀草素、芹菜素和黄芩素-7-甲醚分别在0.88 ～ 176μg/mL、1.1～219.2 μg/mL和1.55～149.4 μg/mL范围内表现出良好的线性(r＞0.999)...

AIM To determine the contents of total and free flavonoids from different tissues of Mosla chinensis in different habitats,and to select and find the raw materials with higher contents and main storage sites of the flavonoids.METHODS Total flavonoids in Mosla chinensis were determined by UV spectrophotometer,and the optimized analysis conditions consisted of concentration of aluminum chloride（0.05 mol/L）,pH 5.0 of acetate buffer with water bath at 50 ℃ and 30 min in detection wavelength of 420 nm.Free flavonoids（luteolin,apigenin and neglet...