Fluorescent-Dye-Labeled Amino Acids for Real-Time Imaging in Arabidopsis thaliana

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成果类型：

期刊论文

作者：

Guangming Yuan;Yao Yuan;Fuxiang Cao*

通讯作者：

Fuxiang Cao

作者机构：

[Guangming Yuan; Yao Yuan] College of Science, Central South University of Forestry and Technology, Changsha 410004, China

College of Horticulture, Hunan Agricultural University, Changsha 410128, China

Author to whom correspondence should be addressed.

[Fuxiang Cao] College of Science, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.

通讯机构：

[Fuxiang Cao] C

College of Science, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.

语种：

英文

关键词：

Arabidopsis thaliana;fluorescent imaging;dye-labeled;amino acid uptake

期刊：

Molecules

ISSN：

1420-3049

年：

2023

卷：

期：

页码：

3126-

DOI：

10.3390/molecules28073126

基金类别：

Conceptualization, methodology, investigation, formal analysis, writing—original draft preparation, Y.Y.; validation, project administration, funding acquisition, supervision, writing—review and editing, F.C.; validation, funding acquisition, supervision, writing—review and editing, G.Y. All authors have read and agreed to the published version of the manuscript. This work was financially supported by The National Natural Science Foundation of China (No. 32171708, 31770606) and the National Key Research and Development Program of China (No. 2019YFE0114600).

机构署名：

本校为通讯机构

院系归属：

园艺园林学院

摘要：

Amino acid is the main transport form of reduced nitrogen in plants. To investigate the uptake and source–sink translocation process of plants to help understand their physiological roles and transport mechanisms, we designed and synthesized three fluorescent-dye-labeled amino acids as tools to visualize amino acid transportation in Arabidopsis thaliana; these amino acids consist of amino acids linked to the fluorophore nitrobenzoxadiazole (NBD) with excellent optical properties. Furthermore, we incubated Arabidopsis thaliana with these NBD fl...

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Fluorescent-Dye-Labeled Amino Acids for Real-Time Imaging in Arabidopsis thaliana

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