期刊论文

Expression of NRAT1 Gene in Hydrangea macrophylla Family

中文

福建农业学报

1008-0384

2019

34

6

646-651

10.19303/j.issn.1008-0384.2019.06.004

湖南省自然科学基金项目（2017JJ3105）；

本校为第一机构

[目的]铝胁迫是酸性土壤中限制植物生长的重要元素,八仙花是一种富集铝的园林观赏植物;本研究对八仙花NRAT1基因进行生物信息学分析及表达分析,以期揭示NRA T1基因在耐铝生理中的作用.[方法]以八仙花品种Hydrangea macrophyllacv laybla为材料,提取RNA进行RT-PCR,检测实时表达情况,克隆NRAT1基因全长并进行生物信息学分析.[结果]试验克隆得到了巨噬基因家族的NRAT1基因全长,该基因有1个1 881 bp的开放阅读框,编码548个氨基酸.多重序列分析表明其具有相当高的保守性,该蛋白有12个跨膜结构,预测位于质膜或液泡膜上,预测结果显示该蛋白是一个疏水性蛋白,试验还分析得到了蛋白质的二、三级结构.RT-P...

【Objective】Bioinformatics and expression of NRAT1 gene of the ornamental Hydrangea macrophylla plants that tend to accumulate aluminum (Al),a growth-limiting heavy metal in acidic soil,were studied to understand the physiology of the plant on Al-tolerance.【Method】The RNA of H.medalensis cv.Laybla was extracted for RT-PCR to detect the real-time expression and cloned for bioinformatic analysis to determine the molecular structure of NRAT1.【Result】 The cloned macrophage gene obtained in the lab revealed that the gene had a 1 881 bp open rea...