期刊论文

The development of ELISA - kit of quantitative analysis for penicillic acid from Penicillium cyclopium

中文

黑龙江畜牧兽医

1004-7034

2010

8

14-16

10.13881/j.cnki.hljxmsy.2010.15.067

国家自然科学基金项目（30671548）；

本校为第一机构

为了建立用于定量检测饲料与食品中青霉酸的ELISA试剂盒,试验在合成青霉酸人工抗原并免疫小鼠获得特异单克隆抗体的基础上,利用间接竞争ELISA方法对试剂盒各项技术参数进行测定,最终获得特异、快速的检测试剂盒.结果表明:在优化的条件下,该试剂盒对青霉酸标准品的最低检测浓度为1.6μg/mL;线性方程为y=9.54x+5.844 4(R

To develop initially the ELISA - kit used to detective quantitatively the penicillic acid in food and feed. On the basic of artificial antigen and monoclonal antibody required through immunizing the nfice,the technical parameters of the kit were determined bv indirect competitive ELISA,ultimately obtaining a special and rapid test kit. Under the optimized conditions,the limited concentration of kit was 1.6 μg/mL,linear equation was y = 9.54x ＋ 5. 844 4 （R^2 =0. 996 4） with linear range of 0.1 - 25.6 μg/mL. The recoveLy rate of kit was 80.5...