期刊论文

中文

分析测试学报

1004-4957

2010

29

12

1130-1134,1142

10.3969/j.issn.1004-4957.2010.12.002

中国博士后科学基金资助项目（20090461007） 湖南省教育厅青年基金资助项目（08B035）

本校为第一机构

在纤维素-三-(3,5-二甲基苯基氨基甲酸酯)(Chiralcel OD-H)手性柱上对茚虫威对映体的分离进行了研究.考察了流动相中改性剂种类和浓度,流速及柱温对分离效果的影响,并对茚虫威对映体与固定相之间保留和分离的热力学机理进行了讨论.结果表明,以正己烷-异丙醇(85∶15)为流动相,柱温为25℃,流速1.0 mL.min-1时,茚虫威对映体能获得基线分离,分离因子(α)和分离度(Rs)分别为1.50和3.49;分别以5种体积分数均为15%的醇改性正己烷,分离因子的变化顺序为:异丁醇

The chiral separation of indoxacarb enantiomers on a Chiralcel OD-H column（cellulose tris（3,5-dimethylphenylcarbamate））was studied by high-performance liquid chromatography（HPLC）.The effects of chromatographic conditions such as the mobile phase composition,concentration and type of alcohol modifier in hexane,flow rate and column temperature on chiral separation of indoxacarb enantiomers were investigated,and the thermodynamic process of the retention and separation between the indoxacarb enantiomers and chiral stationary phase was studie...