期刊论文

中文

分析测试学报

1004-4957

2011

30

7

780-783

10.3969/j.issn.1004-4957.2011.07.013

湖南农业大学校青年基金资助项目（08QN10） 湖南省科技厅计划资助项目（2009NK3107）

本校为第一机构

应用吸附法将羊抗人IgG抗体直接固定于纳米金（GNPs）/壳聚糖（Chit）掺杂碳纳米管（CNTs）修饰的金电极表面,制备了用于人IgG抗原检测的非标记电化学免疫传感器。利用循环伏安法和交流阻抗研究了修饰电极表面的电化学特性,用差分脉冲伏安法研究了测试底液的pH值对免疫传感器性能的影响。实验表明,在含不同浓度人IgG的PBS溶液（pH7.0）中,免疫传感器的响应电流与人IgG的质量浓度在0.05455μg/L范围内呈良好的线性关系,相关系数（r2）为0.991 1,其检出限（S/N=3）为0.01μg/L。与传统方法相比,该免疫传感器无需信号放大试剂,具有制备简单、灵敏度高、稳定性好等特点。

A highly sensitive label-free electrochemical immunosensor was developed for the determination of human IgG based on a gold electrode modified with GNPs and carbon nanotubes（CNTs） incorporated with chitosan composite and the IgG antibody was directly absorbed on the modified electrode surface.The electrochemical behaviors of the modified electrode were investigated by cyclic voltammetry（CV） and AC impedance.The influence of electrolyte pH on immunosensor was studied by differential pulse voltammetry（DPV）.A good linearity was obtained betw...