期刊论文

Separation of Regaloside A,Acetylregaloside C and Regaloside B in Bulbs of Lilium Species by High Speed Counter-current Chromatography and Research on Their Biological Activities

中文

分析测试学报

1004-4957

2018

37

9

1027-1033

10.3969/j.issn.1004-4957.2018.09.007

现代农业产业技术体系建设专项资金资助（CARS-21） 湖南省科技创新计划项目（S2017SFXYZ0116）

本校为第一机构

建立了高速逆流色谱分离制备药食同源植物百合磷茎中3个酚酸甘油酯苷的方法,同时测定了化合物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除作用、脂质的过氧化抑制作用及对α-淀粉酶活性的促进作用。以乙酸乙酯-正丁醇-水(0.5%乙酸,3 ∶ 1.5 ∶ 5,体积比)为溶剂系统,上相为固定相,下相为流动相,从250 mg百合磷茎提取物中分离得到4.2 mg纯度为96.2%的化合物1,2.3 mg纯度为95.1%的化合物2,5.8 mg纯度为98.8%的化合物3。经质谱及核磁共振鉴定化合物1 ~ 3分别为王百合苷A(Regaloside A) 、乙酰化王百合苷C(Acetylregaloside C) 、王百合苷B(Regaloside B);活性研究表明,Acetylregaloside C对DPPH自...

A method of high speed counter-current chromatography was developed for the separation and preparation of three active compounds,phenolic glycerol glycosides from the bulbs of Lilium species used as Chinese tradition medicine. The ethylacetate-n-butanol-water (0.5% acetic acid, 3 ∶ 1.5 ∶ 5,by volume) was used as a two-phase solvent system,in which the upper phase was stationary phase and the lower phase was mobile phase for the isolation of the compounds from the extract of Lilium brownii F. E. Brown var. viridulum Baker by high speed counter...