An efficient method for the preparative isolation and purification of alkaloids from Gelsemium by using high speed counter-current chromatography and preparative HPLC

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成果类型：

期刊论文

作者：

Liang, Ling-Ling;Zhao, Xue-Jiao;Lu, Ying;Zhu, Shi-Hao;Tang, Qi;...

通讯作者：

Liu, ZY

作者机构：

[Zhao, Xue-Jiao; Liang, Ling-Ling; Liu, Zhao-Ying; Zuo, Meng-Ting] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.

[Zhao, Xue-Jiao; Liang, Ling-Ling; Liu, Zhao-Ying; Zuo, Meng-Ting] Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha, Hunan, Peoples R China.

[Lu, Ying; Zhu, Shi-Hao; Tang, Qi] Hunan Agr Univ, Coll Hort & Landscape Architecture, Changsha, Hunan, Peoples R China.

[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Liu, ZY ] H

Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

Alkaloids;Gelsemium elegans;High-speed counter-current chromatography;Koumine;Prep-HPLC combination

期刊：

Preparative Biochemistry & Biotechnology

ISSN：

1082-6068

年：

2024

卷：

期：

页码：

1205-1215

DOI：

10.1080/10826068.2024.2336990

基金类别：

Aid program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

动物医学院

摘要：

We established an efficient method using high-speed countercurrent chromatography (HSCCC) combined with preparative high-performance liquid chromatography (prep-HPLC) for isolating and purifying Gelsemium elegans (G. elegans) alkaloids. First, the two-phase solvent system composed of 1% triethylamine aqueous solution/n-hexane/ethyl acetate/ethanol (volume ratio 4:2:3:2) was employed to separate the crude extract (350 mg) using HSCCC. Subsequently, the mixture that resulted from HSCCC was further separated by Prep-HPLC, resulting in seven pure ...

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An efficient method for the preparative isolation and purification of alkaloids from Gelsemium by using high speed counter-current chromatography and preparative HPLC

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