期刊论文

Hao, Z.Y.

中文

中国寄生虫学与寄生虫病杂志

1000-7423

2006

24

6

445-448

10.3969/j.issn.1000-7423.2006.06.010

湖南农业大学稳定人才基金（03WD07）~~；

本校为第一机构

目的 探讨日本血吸虫SjIR3核酸疫苗诱导小鼠抗日本血吸虫攻击感染的保护效果.方法 用SjIR3的特异引物构建疫苗SjIR3/pC.54只昆明小鼠随机分为3组:A组(对照组)、B组(空质粒组)和C组(实验组)分别肌注生理盐水(100 μl)、pcDNA3.0和SjIR3/pC(各50 μg),共免疫3次,每次间隔2周.于每次免疫前和感染前尾静脉采血,ELISA检测血清IgG抗体水平.末次免疫后2周,各组剖杀其中6只小鼠取脾细胞,分别用ConA或rSjIR3重组蛋白诱导,用四甲基偶氮唑盐微量酶反应比色法(MTT)检测脾淋巴细胞增殖情况.各组余下小鼠分别经腹部感染日本血吸虫尾蚴40±1条,45 d后宰杀,观察实验组小鼠的减虫率和肝减卵率.结果 ELISA结果显示...

OBJECTIVE: To detect the protection in mice induced by DNA vaccine encoding SjIR3 against Schistosoma japonicum (Sj). METHODS: Sj1R3 was amplified by PCR with specific primers and linked to T vector. The reconstructed plasmid was digested by Xho I and BamH I. The target segments were collected and inserted into pcDNA3.0 to construct DNA vaccine SjIR3/pC. Fifty-four female mice were divided into 3 groups: groups A and B received normal saline and pcDNA3.0 respectively as controls, and group C was immunized with SjIR3/pC. All the mice were injected three times with an interval of two weeks. Sera...