期刊论文

Polymorphism of mitochondrial pnad1 gene of Spirometra erinaceieuropaei sparganum isolated from different species of snake in Hunan Province

中文

中国寄生虫学与寄生虫病杂志

1000-7423

2019

37

4

448-452

10.12140/j.issn.1000-7423.2019.04.014

湖南省科技厅项目(No.2016NK2104) 湖南省教育厅优秀青年基金项目(No.17B126) 湖南省畜牧局项目资助(No.2139999).

本校为第一机构

目的 对湖南省不同种类蛇源裂头蚴进行形态学与分子生物学鉴定,并分析其线粒体烟酰胺腺嘌呤二核苷酸脱氢酶第1亚基基因部分序列(pnad1)多态性. 方法 对从湖南永州、郴州、岳阳等地区6种常见的野生蛇体内获得的11条裂头蚴(来自大王蛇3条、乌梢蛇2条、眼镜蛇1条、灰鼠蛇2条、滑鼠蛇2条和银环蛇1条)进行形态学鉴定,PCR扩增其线粒体pnad1,并测序,分析其核苷酸序列种内差异,并与已报道的迭宫属绦虫和其他属绦虫的相应序列进行同源性比较;以土耳其斯坦东毕吸虫为外群,用软件MEGA7.0采用邻接法构建系统进化树. 结果 从不同蛇类分离的11条虫体经形态学鉴定为裂头蚴.PCR扩增出11条裂头蚴分离株的nad1序列...

Objective Spirometra erinaceieuropaei sparganum larvae were isolated from different species of snake and identified by morphology and molecular biology. The polymorphism of partial mitochondrial NADH dehydrogenase subunits 1 gene (pnadl) amplified from these isolated spargana was analyzed for its potential as a species marker. Methods Eleven sparganum larvae of S. erinaceieuropaei were isolated from different species of wild snake [Elaphe carinata (3), Zaocys dhumnade (2), Naja (1), Ptyas korros (2), Ptyas mucosus (2) and Bungarus multicinctus (1)] in Yongzhou, Chenzhou, Yueyang of Hunan Provi...