INTRODUCTION: Research on the molecular basis of dominant male sterility in rice and its application in sterile lines is significantly underdeveloped. This article aims to utilize dominant nuclear male sterile lines, which were created through the ectopic expression of ZmMs7 in the genetic background of rice, for the purpose of heterosis utilization. METHODS: At the same time, we conducted a study on the spatiotemporal expression characteristics of ZmMs7, performed transcriptome analysis, and implemented yeast two-hybrid experiments to elucidate its molecular regulatory mechanisms in mediating...