Optimizing measure for the establishment of regeneration system of callus was given in this paper by using embryonic callus induced from 6 fertilizing parents lines of xian-type hybrid rice mature embryo scutum as explants in this experiment. A regeneration system suitable for rice genetic transformation was built by regulating the concentration of 2, 4-D, medium ingredient, inoculation method, hormones combination and the cell differentiation paths of the callus. The results showed that MB culture media was suitable for callus derivation,and the callus derivation rate in the 6 rice species wa...