期刊论文

中文

现代生物医学进展

1673-6273

2009

9

10

1878-1880

美国孟德尔生物技术公司合作项目

本校为第一机构

芒属植物(Miscanthus)属禾本科多年生高大草类,多分布于热带非洲至亚洲东南部.近年来在欧美国家受到广泛的关注,被认为是一种开发潜力巨大的生物质能源.本文研究了芒属植物染色体制片过程中的几个关键步骤,建立了较成熟的核型分析技术体系:预处理液为0.2%秋水仙素+o.002mol/L8-羟基喹啉混合溶液,常温下处理2h;卡诺氏固定液在4℃下固定20 h;解离步骤为:先用1 mol/L盐酸在60℃下处理15 min,再用2%纤维素酶在28℃下处理1h;再固定,压片,用改良卡宝品红染色.同时,对采自广西柳州的芒进行了核型分析,结果显示其体细胞染色体数目为38条,核型公式是2n=2x=38=32m+6sm,按Stebbins核型标准分类属于2B型.

Miscanthus is a kind of perennial grass with a tall-stalk, mostly distributed in the tropic Africa and the southeast of Asia. Miscanthus has attracted wide interests in the European and American countries in recent years, for it is considered as a new kind of biomass energy source with great developing potential. In this article, several important steps for Miscanthus chromosome observation have been studied, and a sophisticated system for karyotype analysis was developed. The reagent for pretreatment was 0.2% colchicin and 0.002 mol/L 8-hydrox...