期刊论文

中文

现代生物医学进展

1673-6273

2009

9

3

497-499

10.13241/j.cnki.pmb.2009.03.015

湖南省教育厅高等学校科研项目（06C407）； 湖南省科技计划项目（06FJ3143）；

本校为第一机构

目的：通过研究微生物发酵和超声波联合处理对钩藤碱提取率的影响，寻找高效提取钩藤碱的发酵菌株的处理方法。方法：采用微生物发酵与超声波处理中药钩藤粉末，用70％乙醇提取钩藤碱，用紫外分光光度法测定钩藤碱含量。结果：与未经处理相比，单独发酵处理或超声波处理以及发酵与超声波联合处理均能提高钩藤碱的提取率，其中以酵母发酵、酵母发酵与超声波联合处理法提取率最高，分别为对照（未处理）提取效率的2．598倍和2．857倍。结论：酵母是高效提取钩藤碱的优良发酵菌株，酵母发酵与超声波联合处理更显著地提高钩藤碱的提取效率，为钩藤碱的高效提取提供了一种简便、廉价、有效的新方法，...

Objective： To develop a high-throughput effective extraction method for rhynchophylline from the fermentation strain treated by microorganism fermentation combined with ultrasonic. Methods： The uncariar rhynchophylla powder was treated by microorganism fermentation and ultrasonic, then the rhynchophylline was extracted by 70 % ethanol. The contents were measured by uv spectrophotometry. Results： The extraction ratio of rhynchophylline treated by fermentation or ultrasonic treatment or fermentation combined with ultrasonic treatment were high...