期刊论文

中文

现代生物医学进展

1673-6273

2009

9

8

1529-1530+1544

湖南省重大科技专项（07NK1008）；

本校为第一机构

目的:建立妇血荣胶囊中芍药苷含量的HPLC检测方法.方法:采用甲醇提取样品;色谱条件:Alltech ODS柱(5μm,250mm×4.6 mm);流动相为乙腈-水(16:84,v/v);检测波长为230 nm.结果:线性范围为24.3～218.7 μg.mL-1(r=0.9999),平均回收率为101.21%,RSD为0.80%.结论:高效液相色谱法简单易行,准确,灵敏度高,适用于妇血荣胶囊中芍药苷的含量测定.

bjective： To establish the HPLC method for determination of paeoniflorin content in Fuxuerong capsules. Methods： Samples were extracted by methanol. Chromatographic conditions： Alltech ODS （250 mm × 4.6 mm, 5 m）, mobile phase consisting of acetonitrile-water （16：84, v/v） and detected at wavelength of 230nm. Results： The calibration curve for paeoniflorin was linear over the range of 24.3-218.7 g.omL^-1 （r=0.9999） and the recoveries were 101.21% with RSD 0.8%. Conclusion： The established HPLC method is simple, rapid, accurate and sensitive, and can be...